Serum Bilirubin Test

                                                Serum Bilirubin Test

Method: Waller method

Principle: Serum + Sulfanilic acid + sodium nitrite   Reaction   Color complex. Color is measured by a colorimeter/spectrophotometer.

Articles:

1. Colorimeter/spectrophotometer

            2. Test tubes with rack

            3. Micropipette

            4. Reagents:

a. Sulfanilic acid

                                  b. Sodium nitrate acid

                                  c. Sample: Serum/Plasma

           5. Marker, Tissue paper, Cotton etc.

Procedure:                                        

1.     Working reagent   =    0 ml      -    1ml
2.     Bland reagent       =    1 ml      -    0ml
3.     Serum                  =   100 µl    -   100µl
                            Mix and allow to stand for 10 minutes protected frpm light. 
                           Measure  absorbance of sample against the sample blank by     colorimeter /spectrphotometer.

Calculation
                    Test of OD * 12.5
                   =   mg/dl

Normal  range: 0.3 to 1.2  mg/dl